League Two leaders Wycombe Wanderers saw their 10-match unbeaten run ended by Exeter as the Grecians pulled off a shock 2-1 win.
The Chairboys, who made the trip having been unbeaten on the road all season, took the lead through Peter Murphy but David Wheeler and Scot Bennett earned City a rare home win.
"It has been a heck of a run but it has come to an end now so people can stop asking me about how far that can go," Ainsworth said.
"We have been put in a mammoth effort and the lads looked a little bit leggy. Although we had one of our poorer performances, we created some really good chances and that is the positive for me."